| MWexperimental | 150 | kDa |
| MWexpected | 86 | kDa |
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log I(s)
6.32×10-2
6.32×10-3
6.32×10-4
6.32×10-5
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s, nm-1
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Diving into the structural architecture of the B2 SINE ribozyme
Trushar Patel.SASDV73 – B2 SINE ribozyme with deletion mutation 81-124
B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124|
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Fits and models
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Synchrotron SAXS data from solutions of B2del81-124 in 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH 7.9 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 1.5 mg/ml was injected onto a Shodex 403KW-4F HPLC column at 15°C using a flow rate of 0.16 ml/min. 600 successive 3 second frames were collected. Eight successive 3 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Due to the characteristics of the sample, the Porod volume analysis is insufficient.
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