SASDVA4 – β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Size-exclusion chromatography coupled small-angle X-ray scattering (SEC-SAXS) data of B75KN-CTD bound to LILRB3 were obtained using the SIBYLS beamline 12.3.1 at the Advanced Light Source, Lawrence Berkeley National Laboratory. The experiments were conducted with a sample-to-detector distance of 2.1 m and a wavelength. λ, of 0.1127 nm, yielding scattering vectors (s) ranging from 0.1 nm⁻¹ to 4 nm⁻¹. The scattering vector, s, is calculated using the equation s = 4πsinθ/λ, where 2θ represents the scattering angle. Size-exclusion chromatography (SEC) was performed using an Agilent 1260 series HPLC with a Shodex analytical column. A 60 μl sample at a 5 mg/ml protein concentration was injected at a flow rate of 0.65 ml/min onto a Shodex KW-803 column at 4°C. A total of 906 successive 2-second frames were captured. Subsequently, data normalization was conducted against the intensity of the transmitted beam, followed by radial averaging, and subtraction of the scattering from a solvent blank.