| MWI(0) | 385 | kDa |
| MWexpected | 301 | kDa |
| VPorod | 647 | nm3 |
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log I(s)
2.60×10-1
2.60×10-2
2.60×10-3
2.60×10-4
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s, nm-1
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The structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix
Shah A, Zhang X, Snee M, Lockhart-Cairns M, Levy C, Jowitt T, Birchenough H, Dean L, Collins R, Dodd R, Roberts A, Enghild J, Mantovani A, Fontana J, Baldock C, Inforzato A, Richter R, Day A, Matrix Biology 136:52-68 (2025) DOISASDVB6 – Pentraxin-3 truncation mutant lacking the first 24 amino acids per mature promoter
Pentraxin-related protein PTX3 (N-terminal deletion mutant)|
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Fits and models
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Synchrotron SAXS data from solutions of Pentraxin-3 (N-terminal deletion mutant) in 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 8.5 mg/ml was injected at a 0.075 ml/min flow rate onto a Cytiva Superose 6 Increase 3.2/300 column at 20°C. 620 successive 1 second frames were collected throughout the entire SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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