MWexperimental | 107 | kDa |
MWexpected | 107 | kDa |
VPorod | 600 | nm3 |
log I(s)
5.96×10-2
5.96×10-3
5.96×10-4
5.96×10-5
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Synchrotron SAXS
data from solutions of
7SK small nuclear RNA State A Low Magnesium Concentration Ensemble
in
10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH 5.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.094 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample
at 1 mg/ml was injected at a 0.16 ml/min flow rate
onto a Shodex KW403-4F column
at 20°C.
600 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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