Solution Structure Determination and Biophysical Studies of 7SK RNP and 7SL SRP RNAs.

D'Souza MH, Pereira HS, Tersteeg S, Vasudeva G, Siddiqui MQ, Agunlejika T, Kerr L, Girodat D, Patel T, RNA (2025) Europe PMC

SASDVD9 – 7SK small nuclear RNA State B Low Magnesium Concentration Ensemble

RNA component of 7SK nuclear ribonucleoprotein
MWexperimental 107 kDa
MWexpected 107 kDa
VPorod 290 nm3
log I(s) 9.38×10-2 9.38×10-3 9.38×10-4 9.38×10-5
RNA component of 7SK nuclear ribonucleoprotein small angle scattering data  s, nm-1
ln I(s)
RNA component of 7SK nuclear ribonucleoprotein Guinier plot ln 9.38×10-2 Rg: 7.9 nm 0 (7.9 nm)-2 s2
(sRg)2I(s)/I(0)
RNA component of 7SK nuclear ribonucleoprotein Kratky plot 1.104 0 √3 sRg
p(r)
RNA component of 7SK nuclear ribonucleoprotein pair distance distribution function Rg: 7.7 nm 0 Dmax: 24.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
RNA component of 7SK nuclear ribonucleoprotein DAMMIN model
log I(s)
 s, nm-1
RNA component of 7SK nuclear ribonucleoprotein DAMMIN model
log I(s)
 s, nm-1
RNA component of 7SK nuclear ribonucleoprotein DAMMIN model
Synchrotron SAXS data from solutions of 7SK small nuclear RNA State B Low Magnesium Concentration Ensemble in 10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH 5.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RNA component of 7SK nuclear ribonucleoprotein (7SK snRNA)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   106.7 kDa
Sequence   FASTA