| MWI(0) | 35 | kDa |
| MWexpected | 42 | kDa |
| VPorod | 55 | nm3 |
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log I(s)
2.47×10-2
2.47×10-3
2.47×10-4
2.47×10-5
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s, nm-1
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Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky.SASDVS8 – Glucosidase/Rutinosidase from Aspergillus niger - deglycosylated
glucan 1,3-beta-glucosidase (W90L, N179H)|
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Fits and models
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SAXS data from solutions of Glucosidase/Rutinosidase (deglycosylated) in McIlvain (Na2HPO4/citric acid) buffer, pH 3.5 were collected using an Anton Paar SAXSpoint 2.0 instrument at the Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure (Vestec, Czech Republic) equipped with an Eiger R 1M detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.40 mg/ml was measured at 20°C. 40 successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Glucosidase/Rutinosidase from Aspergillus niger was treated with endo H glycosidase. Raw data are available at: https://doi.org/10.5281/zenodo.13950823 |
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