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Synchrotron SAXS data from solutions of Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 14 as the linker region in 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 6 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-800 series column at 7°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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s, nm-1
