The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family

Jens Reiners.

SASDW27 – ABC transporter ATP-binding protein - ABC transporter, permease protein - complex from S.agalactiae (SaNsrFP)

ABC transporter ATP-binding protein
ABC transporter, permease protein
MWexperimental 148 kDa
MWexpected 136 kDa
VPorod 735 nm3
log I(s) 1.48×102 1.48×101 1.48×100 1.48×10-1
ABC transporter ATP-binding protein ABC transporter, permease protein small angle scattering data  s, nm-1
ln I(s)
ABC transporter ATP-binding protein ABC transporter, permease protein Guinier plot ln 1.48×102 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
ABC transporter ATP-binding protein ABC transporter, permease protein Kratky plot 1.104 0 √3 sRg
p(r)
ABC transporter ATP-binding protein ABC transporter, permease protein pair distance distribution function Rg: 6.0 nm 0 Dmax: 20.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ABC transporter ATP-binding protein - ABC transporter, permease protein - complex from S.agalactiae (SaNsrFP) in 50 mM Tris, 300 mM NaCl, 10 % Glycerol, 0.005 % LMNG,, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ABC transporter ATP-binding protein (SaNsrF)
Mol. type   Protein
Organism   Streptococcus agalactiae
Olig. state   Dimer
Mon. MW   30.9 kDa
 
UniProt   X5KGL2 (1-250)
Sequence   FASTA
 
ABC transporter, permease protein (SaNsrP)
Mol. type   Protein
Organism   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Olig. state   Monomer
Mon. MW   74.0 kDa
 
UniProt   Q8DZX0 (1-651)
Sequence   FASTA