Synchrotron SAXS data from solutions of PPARα-AB in 25 mM HEPES, 150 mM NaCl, 5 mM NaNO₃, 5 mM TCEP, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123972 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 10 mg/ml was injected at a 0.70 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 23°C. 58 successive 1 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The quoted experimental molecular weight was determined using multi angle laser light scattering and refractive index measurements (MALLS/RI). The molecular weight estimated from the SAXS data using Bayesian inference spans 14–17 kDa. The MALLS/RI data in addition to the unsubtracted SEC-SAXS data frames collected throughout the entire SEC elution (2100 data frames) are made available in the full entry zip archive.
s, nm-1
