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Rational design of biosensors by computational analysis

Jens Reiners.

SASDWP9 – Ratiometric matryoshka biosensor for Fe2+ – MDtxRGA_G149

Ratiometric matryoshka biosensor for Fe2+
MWexperimental 83 kDa
MWexpected 84 kDa
VPorod 118 nm3
log I(s) 5.83×100 5.83×10-1 5.83×10-2 5.83×10-3
Ratiometric matryoshka biosensor for Fe2+ small angle scattering data  s, nm-1
ln I(s)
Ratiometric matryoshka biosensor for Fe2+ Guinier plot ln 5.83×100 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Ratiometric matryoshka biosensor for Fe2+ Kratky plot 1.104 0 √3 sRg
p(r)
Ratiometric matryoshka biosensor for Fe2+ pair distance distribution function Rg: 3.6 nm 0 Dmax: 12.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ratiometric matryoshka biosensor for Fe2+ ALPHAFOLD model
Synchrotron SAXS data from solutions of Ratiometric matryoshka biosensor for Fe2+ (MDtxRGA_G149) in 20 mM MOPS, 250 mM KCl, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 4 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 1200 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ratiometric matryoshka biosensor for Fe2+ (MDtxRGA G149)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   84.2 kDa
Sequence   FASTA