Alu RNA pseudoknot alterations influence SRP9/SRP14 association.

Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA, RNA (2025) Europe PMC

SASDWR3 – BC120 G25C RNA

BC120 G25C RNA
MWexperimental 45 kDa
MWexpected 39 kDa
VPorod 63 nm3
log I(s) 5.75×10-2 5.75×10-3 5.75×10-4 5.75×10-5
BC120 G25C RNA small angle scattering data  s, nm-1
ln I(s)
BC120 G25C RNA Guinier plot ln 5.76×10-2 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
BC120 G25C RNA Kratky plot 1.104 0 √3 sRg
p(r)
BC120 G25C RNA pair distance distribution function Rg: 4.3 nm 0 Dmax: 13.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
BC120 G25C RNA DAMMIN model
log I(s)
 s, nm-1
BC120 G25C RNA DAMMIN model
log I(s)
 s, nm-1
BC120 G25C RNA DAMMIN model
Synchrotron SAXS data from solutions of BC120 G25C RNA in phosphate buffered saline, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 3 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 25°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

BC120 G25C RNA
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   38.9 kDa
Sequence   FASTA