The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold

Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E, Plant Physiology (2025) DOI

SASDWS3 – N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)

N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)
MWexperimental 96 kDa
MWexpected 97 kDa
VPorod 170 nm3
log I(s) 6.47×10-2 6.47×10-3 6.47×10-4 6.47×10-5
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) small angle scattering data  s, nm-1
ln I(s)
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Guinier plot ln 6.48×10-2 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Kratky plot 1.104 0 √3 sRg
p(r)
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) pair distance distribution function Rg: 3.6 nm 0 Dmax: 13.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Rg histogram Rg, nm
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) EOM/RANCH model
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) EOM/RANCH model
N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) EOM/RANCH model
SAXS data from solutions of ArgC in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on a Xenocs Xeuss 2.0 Q-Xoom instrument at the Center for Structural Studies, Heinrich-Heine-University (Düsseldorf, Germany) using a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 10 mg/ml were measured at 10°C. 18 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) (crTPpart2_ArgC+ArgC)
Mol. type   Protein
Organism   Paulinella chromatophora
Olig. state   Dimer
Mon. MW   48.4 kDa
Sequence   FASTA