The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold

Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E, Plant Physiology (2025) DOI

SASDWT3 – crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)

crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)
MWexperimental 13 kDa
MWexpected 14 kDa
VPorod 27 nm3
log I(s) 8.92×10-3 8.92×10-4 8.92×10-5 8.92×10-6
crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) small angle scattering data  s, nm-1
ln I(s)
crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Guinier plot ln 8.93×10-3 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Kratky plot 1.104 0 √3 sRg
p(r)
crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) CORAL model
Synchrotron SAXS data from solutions of crTPpart2 of ArgC in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.6 and 8.6 mg/ml were measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) (crTPpart2_ArgC)
Mol. type   Protein
Organism   Paulinella chromatophora
Olig. state   Monomer
Mon. MW   14.0 kDa
Sequence   FASTA