Synchrotron SAXS data from solutions of PPARγ in 20 mM HEPES, 150 mM KCl, 1 mM TCEP, 2% v/v glycerol, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 8 mg/ml was injected at a 0.35 ml/min flow rate onto a Cytiva Superdex 200 Increase 5/150 column at 23°C. 64 successive 0.250 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The quoted experimental molecular weight was determined using multi angle laser light scattering and refractive index measurements (MALLS/RI). The molecular weight estimated from the SAXS data using Bayesian inference spans 56–65 kDa. The MALLS/RI data in addition to the unsubtracted SEC-SAXS data frames collected throughout the entire SEC elution (2880 data frames) are made available in the full entry zip archive.
s, nm-1
