Structural and functional characterization of VapBC52 toxin-antitoxin system from Mycobacterium tuberculosis.

Singh M Singh C, Nair AV, Ahmad I, Sharma A, Bhasin M, Jain V, Singh R, Thakur KG, Nucleic Acids Res 54(11) (2026) Europe PMC

SASDX27 – Rv2514c VapC52

Ribonuclease
MWI(0) 38 kDa
MWexpected 38 kDa
VPorod 50 nm3
log I(s) 4.12×101 4.12×100 4.12×10-1 4.12×10-2
Ribonuclease small angle scattering data  s, nm-1
ln I(s)
Ribonuclease Guinier plot ln 4.13×101 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Ribonuclease Kratky plot 1.104 0 √3 sRg
Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ribonuclease GASBOR model
Synchrotron SAXS data from solutions of Rv2514c VapC52 in 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.99 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 1 mg/ml was injected at a 0.75 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 700 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ribonuclease (VapC52)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Dimer
Mon. MW   19.2 kDa
Sequence   FASTA