Structural and functional characterization of VapBC52 toxin-antitoxin system from Mycobacterium tuberculosis.

Singh M, Singh C, Nair AV, Ahmad I, Sharma A, Bhasin M, Jain V, Singh R, Thakur KG
Nucleic Acids Res 54(11) (2026 Jun 8)
PMID: 42306950
doi: 10.1093/nar/gkag611 		Submitted to SASBDB: 2025 Jul 28
Published in SASBDB:

SASDX27 – Rv2514c VapC52

Ribonuclease experimental SAS data
GASBOR model
Sample: Ribonuclease dimer, 38 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 5
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 50 nm3

SASDX37 – Rv2514c-Rv2515c VapBC52 complex

Ribonuclease VapC52Antitoxin VapB52 experimental SAS data
GASBOR model
Sample: Ribonuclease VapC52 dimer, 37 kDa Mycobacterium tuberculosis protein
Antitoxin VapB52 monomer, 43 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 5
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 107 nm3