Non Structural Protein 15 in Complex with ADPRP Macrodomain of SARS CoV 2

Hira Singh Gariya.

SASDX29 – Non Structural Protein 15 and ADPRP Macrodomain D

Non structural Protein 15 in Complex with ADPRP Macrodomain
MWI(0) 208 kDa
MWexpected 254 kDa
log I(s) 2.38×104 2.38×103 2.38×102 2.38×101
Non structural Protein 15 in Complex with ADPRP Macrodomain small angle scattering data  s, nm-1
ln I(s)
Non structural Protein 15 in Complex with ADPRP Macrodomain Guinier plot ln 2.38×104 Rg: 6.1 nm 0 (6.1 nm)-2 s2
(sRg)2I(s)/I(0)
Non structural Protein 15 in Complex with ADPRP Macrodomain Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

SAXS data from solutions of Non Structural Protein 15 and ADPRP Macrodomain D in 20mM HEPES, 150mM Nacl, pH 7.5 were collected on the Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India) using a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 1.54 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. Three successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Non structural Protein 15 in Complex with ADPRP Macrodomain (NSP15)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   254 kDa
 
UniProt   P0DTD1 (6465-6798)
Sequence   FASTA