Structural and functional characterization of VapBC52 toxin-antitoxin system from Mycobacterium tuberculosis.

Singh M Singh C, Nair AV, Ahmad I, Sharma A, Bhasin M, Jain V, Singh R, Thakur KG, Nucleic Acids Res 54(11) (2026) Europe PMC

SASDX37 – Rv2514c-Rv2515c VapBC52 complex

Ribonuclease VapC52
Antitoxin VapB52
MWI(0) 83 kDa
MWexpected 80 kDa
VPorod 107 nm3
log I(s) 9.94×101 9.94×100 9.94×10-1 9.94×10-2
Ribonuclease VapC52 Antitoxin VapB52 small angle scattering data  s, nm-1
ln I(s)
Ribonuclease VapC52 Antitoxin VapB52 Guinier plot ln 9.94×101 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Ribonuclease VapC52 Antitoxin VapB52 Kratky plot 1.104 0 √3 sRg
p(r)
Ribonuclease VapC52 Antitoxin VapB52 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ribonuclease VapC52 Antitoxin VapB52 GASBOR model
Synchrotron SAXS data from solutions of Rv2514c-Rv2515c VapBC52 complex in 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 700 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ribonuclease VapC52 (VapC52)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Dimer
Mon. MW   18.6 kDa
Sequence   FASTA
 
Antitoxin VapB52 (VapB52)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Monomer
Mon. MW   43.3 kDa
Sequence   FASTA