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Synchrotron SAXS data from solutions of Pex5 in 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124026 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 77.00 μl sample at 8 mg/ml was injected at a 0.65 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 59 successive 1 second frames were collected through the main SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The experimental molecular weight was estimated from combined multi-angle laser light scattering (MALLS) and refractive index (RI) measurements. These MALLS/RI data, in addition to the unsubtracted SEC-SAXS data frames, MultiFoXS ensemble modelling, and final OLIGOMER volume-fraction weighted fit are made available in the full entry zip archive. The models displayed in this entry represent the volume-fraction weighted ensemble of Pex5 (top), the Rg frequency of each model in the limited ensemble 9-member pool (middle), and an individual model example (most frequent representative, Vf = 0.45).
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s, nm-1
![Peroxisomal targeting signal receptor OTHER [STATIC IMAGE] model](/media//pdb_file/SASDX84_fit1_model1.png "Static model image")
![Peroxisomal targeting signal receptor OTHER [STATIC IMAGE] model](/media//pdb_file/SASDX84_fit1_model2.png "Static model image")
