Synchrotron SAXS
data from solutions of
Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 point mutation (L49V)
in
20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.02% NaN3, pH 7.5
were collected
on the
13A beam line
at the Taiwan Photon Source, NSRRC storage ring
(Hsinchu, Taiwan)
using a Eiger X 1M and Eiger X 9M detector
at a sample-detector distance of 9 m and
at a wavelength of λ = 0.0826 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample
at 12 mg/ml was injected at a 0.10 ml/min flow rate
onto a Agilent Bio SEC-3, 300 Å column
at 10°C.
11 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
