Allosteric network of dynamic coupling within BAP1-UCH revealed by methyl NMR.

Lai CH, Lou YC, Chang CF, Lu WL, Sriramoju MK, Wang YS, Wu KP, Camilloni C, Hsu SD
Nat Commun (2026 May 26)
PMID: 42191708
doi: 10.1038/s41467-026-73600-4 		Submitted to SASBDB: 2025 Jul 10
Published in SASBDB:

SASDXD6 – Ubiquitin C-terminal hydrolase (UCH) domain of BAP1

Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 wild type experimental SAS data
Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 wild type Kratky plot
Sample: Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 wild type monomer, 27 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3 mM CaCl2, 0.02% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2024 Jun 23
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 35 nm3

SASDXE6 – Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 point mutation (L49V)

Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 L49V experimental SAS data
Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 L49V Kratky plot
Sample: Ubiquitin C-terminal hydrolase (UCH) domain of BAP1 L49V monomer, 27 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.02% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2024 Jun 23
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 36 nm3