Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.

Rumpret M, Lewis Marffy AL, Zhang Y van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ, Nat Commun (2026) Europe PMC

SASDXH8 – Leukocyte Immunoglobulin-Like Receptor LILR-B3

Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)
MWexperimental 58 kDa
MWexpected 47 kDa
VPorod 137 nm3
log I(s) 2.75×101 2.75×100 2.75×10-1 2.75×10-2
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) small angle scattering data  s, nm-1
ln I(s)
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) Guinier plot ln 2.76×101 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) Kratky plot 1.104 0 3 sRg
p(r)
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) pair distance distribution function Rg: 3.8 nm 0 Dmax: 13.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) MODELLER model

Synchrotron small-angle X-ray scattering (SAXS) data on Leukocyte Immunoglobulin-Like Receptor (LILR-B3) were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.

Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) (LILRB3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   47.0 kDa
 
UniProt   A0A0G2JM46 (24-443)
Sequence   FASTA