Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2.

Liloku P, Melo DA, Gisbert VG, Mannaert S, Mechleb K, Derclaye S, Ray A, Alsteens D, Volkov AN, Sterckx YG Claeys Bouuaert C, Nucleic Acids Res 54(15) (2026) Europe PMC

SASDXZ2 – Recombination protein 107-COMPASS component SPP1 (Mer2(138-224) - MBPSpp1(161-353)

Recombination protein 107
COMPASS component SPP1
MWexperimental 183 kDa
MWexpected 174 kDa
VPorod 336 nm3
log I(s) 2.63×100 2.63×10-1 2.63×10-2 2.63×10-3
Recombination protein 107 COMPASS component SPP1 small angle scattering data  s, nm-1
ln I(s)
Recombination protein 107 COMPASS component SPP1 Guinier plot ln 2.64×100 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Recombination protein 107 COMPASS component SPP1 Kratky plot 1.104 0 √3 sRg
p(r)
Recombination protein 107 COMPASS component SPP1 pair distance distribution function Rg: 5.5 nm 0 Dmax: 20.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Recombination protein 107 COMPASS component SPP1 XPLOR-NIH model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Recombination protein 107-COMPASS component SPP1 (Mer2(138-224) - MBPSpp1(161-353) in 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 3 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 15°C. 936 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Recombination protein 107 (Mer2(138-224))
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Tetramer
Mon. MW   10.2 kDa
 
UniProt   P21651
Sequence   FASTA
 
COMPASS component SPP1 (MBP-Spp1(161-353))
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Dimer
Mon. MW   66.7 kDa
 
UniProt   Q03012
Sequence   FASTA