Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2.

Liloku P, Melo DA, Gisbert VG, Mannaert S, Mechleb K, Derclaye S, Ray A, Alsteens D, Volkov AN, Sterckx YG, Claeys Bouuaert C
Nucleic Acids Res 54(15) (2026 Aug 10)

PMID: 42626841 doi: 10.1093/nar/gkag785

Submitted to SASBDB: 2025 Mar 22 Published in SASBDB: 2026 Aug 24

SASDXW2 – Recombination protein 107-COMPASS component SPP1 (HisSUMOMer2 - MBPSpp1) Sample: Recombination protein 107 tetramer, 200 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 171 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 10.1 nm Dmax 36.6 nm VolumePorod 649 nm3

SASDXX2 – Recombination protein 107-COMPASS component SPP1 (Mer2(41-224) - Spp1) Sample: Recombination protein 107 tetramer, 84 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 83 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 6.9 nm Dmax 29.7 nm VolumePorod 255 nm3

SASDXY2 – Recombination protein 107-COMPASS component SPP1 (Mer2(41-224) - Spp1(161-353) Sample: Recombination protein 107 tetramer, 84 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 46 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 7.5 nm Dmax 32.0 nm VolumePorod 257 nm3

SASDXZ2 – Recombination protein 107-COMPASS component SPP1 (Mer2(138-224) - MBPSpp1(161-353) Sample: Recombination protein 107 tetramer, 41 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 133 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 5.5 nm Dmax 20.0 nm VolumePorod 336 nm3

SASDYC2 – Recombination protein 107-COMPASS component SPP1 (Mer2(41-224) - Spp1) Sample: Recombination protein 107 tetramer, 84 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 83 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 6.9 nm Dmax 29.7 nm VolumePorod 255 nm3

SASDYD2 – Recombination protein 107-COMPASS component SPP1 (Mer2(41-224) - Spp1(161-353) Sample: Recombination protein 107 tetramer, 84 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 46 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 7.5 nm Dmax 32.0 nm VolumePorod 257 nm3

SASDYE2 – Recombination protein 107-COMPASS component SPP1 (Mer2(138-224) - MBPSpp1(161-353) Sample: Recombination protein 107 tetramer, 41 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 133 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 5.5 nm Dmax 20.0 nm VolumePorod 336 nm3

SASDYF2 – Recombination protein 107-COMPASS component SPP1 (HisSUMOMer2 - MBPSpp1) Sample: Recombination protein 107 tetramer, 200 kDa Saccharomyces cerevisiae (strain … protein COMPASS component SPP1 dimer, 171 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 10.1 nm Dmax 36.6 nm VolumePorod 649 nm3