Human lncRNA RMRP interacts with DEAD-box helicases and modulates mitochondrial function.

Pereira HS, Luddu J, Veerareddygari GR, Sanghvi SK, Patel PB, Robinson ZE, Siddiqui MQ, Singh H, Patel TR, Proc Natl Acad Sci U S A 123(8):e2522583123 (2026) Europe PMC

SASDYE4 – RNA component of mitochondrial RNA processing endoribonuclease (RMRP in 20 mM Mg2+)

RMRP
MWexperimental 108 kDa
MWexpected 91 kDa
VPorod 205 nm3
log I(s) 4.64×10-2 4.64×10-3 4.64×10-4 4.64×10-5
RMRP small angle scattering data  s, nm-1
ln I(s)
RMRP Guinier plot ln 4.64×10-2 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
RMRP Kratky plot 1.104 0 √3 sRg
p(r)
RMRP pair distance distribution function Rg: 5.8 nm 0 Dmax: 18 nm

Data validation

Fits and models

log I(s)
 s, nm-1
RMRP DAMMIN model
log I(s)
 s, nm-1
RMRP DAMMIN model
log I(s)
 s, nm-1
RMRP DAMMIN model
Synchrotron SAXS data from solutions of RNA component of mitochondrial RNA processing endoribonuclease (RMRP in 20 mM Mg2+) in 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, pH 6.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 52.00 μl sample at 1 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 25°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RMRP (RMRP)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   90.5 kDa
Sequence   FASTA