Liloku P,
Melo DA,
Gisbert VG,
Mannaert S,
Mechleb K,
Derclaye S,
Ray A,
Alsteens D,
Volkov AN,
Sterckx YG
Claeys Bouuaert C,
Nucleic Acids Res
54(15)
(2026)
Europe PMC
SASDYF2 – Recombination protein 107-COMPASS component SPP1 (HisSUMOMer2 - MBPSpp1)
Synchrotron SAXS
data from solutions of
Recombination protein 107-COMPASS component SPP1 (HisSUMOMer2 - MBPSpp1)
in
25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM TCEP, pH 7.5
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.099 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample
at 3 mg/ml was injected at a 0.20 ml/min flow rate
onto a Shodex KW404-4F column
at 15°C.
936 successive
0.990 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
