ATPLyzer – An advanced ratiometric multi-colour biosensor for long-term monitoring of ATP dynamics

Jens Reiners.

SASDYQ9 – ATPLyzer GO 1.7 m apo

Ratiometric matryoshka multi-colour biosensor for ATP
MWexperimental 71 kDa
MWexpected 72 kDa
VPorod 110 nm3
log I(s) 3.15×101 3.15×100 3.15×10-1 3.15×10-2
Ratiometric matryoshka multi-colour biosensor for ATP small angle scattering data  s, nm-1
ln I(s)
Ratiometric matryoshka multi-colour biosensor for ATP Guinier plot ln 3.16×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ratiometric matryoshka multi-colour biosensor for ATP Kratky plot 1.104 0 √3 sRg
p(r)
Ratiometric matryoshka multi-colour biosensor for ATP pair distance distribution function Rg: 4.0 nm 0 Dmax: 14.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ratiometric matryoshka multi-colour biosensor for ATP EOM/RANCH model
Synchrotron SAXS data from solutions of ATPLyzer GO 1.7 m apo in 50 mM MOPS, 100 mM KCl, 1 mM MgCl2,, pH 7 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 4.9 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 10°C. 800 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Please read the molecule description by clicking the molecule link

Ratiometric matryoshka multi-colour biosensor for ATP (ATPLyzer GO 1.7m apo)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   72.2 kDa
Sequence   FASTA