| MWexperimental | 72 | kDa |
| MWexpected | 72 | kDa |
| VPorod | 106 | nm3 |
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log I(s)
3.03×101
3.03×100
3.03×10-1
3.03×10-2
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s, nm-1
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ATPLyzer – An advanced ratiometric multi-colour biosensor for long-term monitoring of ATP dynamics
Jens Reiners.SASDYR9 – ATPLyzer GO 1.7 m ATP
Ratiometric matryoshka multi-colour biosensor for ATP|
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Fits and models
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Synchrotron SAXS
data from solutions of
ATPLyzer GO 1.7 m ATP
in
50 mM MOPS, 100 mM KCl, 1 mM MgCl2, 5mM ATP,, pH 7
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus3 2M detector
at a sample-detector distance of 2.8 m and
at a wavelength of λ = 0.099 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 4.9 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 10°C.
800 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Please read the molecule description by clicking the molecule link |
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