Conserved dimerization architecture in C-type lectins from virus-vector mosquitoes

Max Renner.

SASDZ66 – C-type lectin mosGCTL-1 from Aedes aegypti, ~0.8 mg/ml

C-type lectin mosGCTL-1
MWexperimental 31 kDa
MWexpected 35 kDa
log I(s) 2.00×10-2 2.00×10-3 2.00×10-4 2.00×10-5
C-type lectin mosGCTL-1 small angle scattering data  s, nm-1
ln I(s)
C-type lectin mosGCTL-1 Guinier plot ln 2.00×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
C-type lectin mosGCTL-1 Kratky plot 1.104 0 3 sRg

Data validation


Fits and models


log I(s)
 s, nm-1
C-type lectin mosGCTL-1 from Aedes aegypti, ~0.8 mg/ml Rg histogram Rg, nm
C-type lectin mosGCTL-1 GROMACS model
C-type lectin mosGCTL-1 GROMACS model
C-type lectin mosGCTL-1 GROMACS model

Synchrotron SAXS data from solutions of C-type lectin mosGCTL-1 from Aedes aegypti, ~0.8 mg/ml in 25 mM Citrate, 1 M NaCl, 10 mM CaCl2, pH 4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.80 mg/ml was measured at 15°C. 120 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray Exposure time = UNKNOWN

C-type lectin mosGCTL-1
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   17.3 kDa
 
UniProt   Q17NZ6 (18-160)
Sequence   FASTA