Conserved dimerization architecture in C-type lectins from virus-vector mosquitoes

Max Renner.

SASDZ86 – C-type lectin mosGCTL-3 from Aedes aegypti, ~0.45 mg/ml

mosGCTL-3
MWexperimental 40 kDa
MWexpected 36 kDa
log I(s) 1.38×10-2 1.38×10-3 1.38×10-4 1.38×10-5
mosGCTL-3 small angle scattering data  s, nm-1
ln I(s)
mosGCTL-3 Guinier plot ln 1.38×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
mosGCTL-3 Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
C-type lectin mosGCTL-3 from Aedes aegypti, ~0.45 mg/ml Rg histogram Rg, nm
mosGCTL-3 GROMACS model
mosGCTL-3 GROMACS model
mosGCTL-3 GROMACS model
Synchrotron SAXS data from solutions of C-type lectin mosGCTL-3 from Aedes aegypti, ~0.45 mg/ml in 20 mM Tris, 250 mM NaCl, 2.5 mM CaCl2, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.45 mg/ml was measured at 15°C. 20 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray Exposure time = UNKNOWN

mosGCTL-3
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   18.1 kDa
Sequence   FASTA