Synchrotron SAXS
data from solutions of
Polyclonal antibody anti-SmVAL11
in
25 mM MES, 125 mM NaCl, 1 mM EDTA, pH 6.8
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.76 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 2.7 mg/ml was injected at a 0.30 ml/min flow rate
onto a column
at 20°C.
2600 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Polyclonal antibody anti-SmVAL11 alone analyzed by SEC-SAXS.
s, nm-1
