Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst
Markova K,
Chmelova K,
Marques S,
Carpentier P,
Bednar D,
Damborsky J,
Marek M
Chemical Science
11(41):11162-11178
(2020)
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| Sample: |
Haloalkane dehalogenase variant DhaA115 -monomeric fraction monomer, 34 kDa Rhodococcus rhodochrous protein
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| Buffer: |
50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH: 7.5 |
| Experiment: |
SAXS
data collected at Rigaku BioSAXS-1000, CEITEC on 2019 Aug 22
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| RgGuinier |
1.9 |
nm |
| Dmax |
6.0 |
nm |
| VolumePorod |
41 |
nm3 |
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| Sample: |
Haloalkane dehalogenase variant DhaA115 - dimeric fraction dimer, 69 kDa Rhodococcus rhodochrous protein
|
| Buffer: |
50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH: 7.5 |
| Experiment: |
SAXS
data collected at Rigaku BioSAXS-1000, CEITEC on 2019 Aug 22
|
|
| RgGuinier |
2.9 |
nm |
| Dmax |
8.9 |
nm |
| VolumePorod |
78 |
nm3 |
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