The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells

Zhang J, Gurusaran M, Fujiwara Y, Zhang K, Echbarthi M, Vorontsov E, Guo R, Pendlebury D, Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H
Nature Communications 11(1) (2020 Apr 28)

doi: 10.1038/s41467-020-15954-x

Submitted to SASBDB: 2020 Mar 30 Published in SASBDB: 2020 Apr 28

SASDH99 – Meiotic localizer of BRCA2 bound to BRME1 - MEILB2a1+2-BRME1 2:2 complex Sample: BRME1 dimer, 23 kDa Mus musculus protein Meiotic localizer of BRCA2 dimer, 26 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7 RgGuinier 5.0 nm Dmax 19.0 nm VolumePorod 104 nm3

SASDHA9 – Meiotic localizer of BRCA2 - MEILB2a1+2 dimer Sample: Meiotic localizer of BRCA2 dimer, 26 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7 RgGuinier 4.6 nm Dmax 16.0 nm VolumePorod 88 nm3

SASDHB9 – Meiotic localizer of BRCA2 - MEILB2a1+2 octamer Sample: Meiotic localizer of BRCA2 octamer, 102 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7 RgGuinier 5.7 nm Dmax 20.0 nm VolumePorod 368 nm3

SASDHC9 – Meiotic localizer of BRCA2 - MEILB1a2 monomer Sample: Meiotic localizer of BRCA2 monomer, 9 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 43 nm3