Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9

Bochel A, Williams C, McCoy A, Hoppe H, Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
Structure (2020 Sep)

doi: 10.1016/j.str.2020.08.002

Submitted to SASBDB: 2020 Mar 26 Published in SASBDB: 2020 Aug 13

SASDH79 – Human Cation-independent mannose-6-phosphate receptor domains 9-10 Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25 RgGuinier 3.3 nm Dmax 9.2 nm VolumePorod 93 nm3

SASDH59 – Human Cation-independent mannose-6-phosphate receptor domains 9-10 wih mannose-6-phosphate Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25 RgGuinier 3.3 nm Dmax 9.1 nm VolumePorod 88 nm3

SASDH69 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3 RgGuinier 2.5 nm Dmax 7.8 nm VolumePorod 48 nm3

SASDJ23 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated with mannose-6-phosphate Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3 RgGuinier 2.5 nm Dmax 7.8 nm VolumePorod 69 nm3