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11 hits found for Winter

SASDJ43 – Acyl carrier protein-thioesterase domain from Men2

Menisporopsin singlet acyl carrier protein-thioesterase experimental SAS data
Acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin singlet acyl carrier protein-thioesterase monomer, 45 kDa Menisporopsin theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
...Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 3.6 nm
Dmax 14.4 nm
VolumePorod 75 nm3

SASDJ53 – Doublet acyl carrier protein-thioesterase domain from Men2

Menisporopsin doublet Acyl Carrier Protein-Thioesterase experimental SAS data
Doublet acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin doublet Acyl Carrier Protein-Thioesterase monomer, 58 kDa Menisporopsis theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
...Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 4.2 nm
Dmax 17.3 nm
VolumePorod 84 nm3

SASDD45 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein

Type II toxin-antitoxin system HicB family antitoxin experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 28
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 112 nm3

SASDD55 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA

Type II toxin-antitoxin system HicB family antitoxinAddiction module toxin, HicA experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Addiction module toxin, HicA monomer, 7 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 27
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 110 nm3

SASDJ49 – Apo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Apo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
...Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 83 nm3

SASDJ59 – Holo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Holo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
...Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 72 nm3

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data
ALLOSMOD model
Sample: Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein
Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein
Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 8
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
...Winter H, Beyaert R, Lippens G, Savvides SN
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 98 nm3

SASDH79 – Human Cation-independent mannose-6-phosphate receptor domains 9-10

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 93 nm3

SASDH59 – Human Cation-independent mannose-6-phosphate receptor domains 9-10 wih mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.1 nm
VolumePorod 88 nm3

SASDH69 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 48 nm3

SASDJ23 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated with mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 69 nm3