SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase

Bunnak W, Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
FEBS Letters (2020 Oct 26)

doi: 10.1002/1873-3468.13954
Submitted to SASBDB: 2020 May 11
Published in SASBDB:

SASDJ43 – Acyl carrier protein-thioesterase domain from Men2

Menisporopsin singlet acyl carrier protein-thioesterase experimental SAS data
Acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin singlet acyl carrier protein-thioesterase monomer, 45 kDa Menisporopsin theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
RgGuinier 3.6 nm
Dmax 14.4 nm
VolumePorod 75 nm3

SASDJ53 – Doublet acyl carrier protein-thioesterase domain from Men2

Menisporopsin doublet Acyl Carrier Protein-Thioesterase experimental SAS data
Doublet acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin doublet Acyl Carrier Protein-Thioesterase monomer, 58 kDa Menisporopsis theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
RgGuinier 4.2 nm
Dmax 17.3 nm
VolumePorod 84 nm3