Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures

Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
Biophysical Journal (2020 Nov)

doi: 10.1016/j.bpj.2020.10.038 		Submitted to SASBDB: 2020 Sep 5
Published in SASBDB:

SASDJV7 – pri-miR16-1 primary microRNA

primary microRNA pri-miR16-1 experimental SAS data
DAMMIF model
Sample: Primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 74 nm3

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: Primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3