Search

  
Advanced search  

65 hits found for Pabit

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.5 nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data
A dENE + Poly(A)30 Kratky plot
Sample: A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 13.3 nm

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

B dENE experimental SAS data
B dENE Kratky plot
Sample: B dENE monomer, 24 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.8 nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data
B dENE + Poly(A)30 Kratky plot
Sample: B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

C dENE experimental SAS data
C dENE Kratky plot
Sample: C dENE monomer, 29 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.4 nm
Dmax 12.4 nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Xtal dENE experimental SAS data
Xtal dENE Kratky plot
Sample: Xtal dENE monomer, 28 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.1 nm
Dmax 11.0 nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDRE3 – Tissue Transglutaminase + Ca: Time-resolved 0ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 150 nm3

SASDRF3 – Tissue Transglutaminase + Ca: Time-resolved 32 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 170 nm3

SASDRG3 – Tissue Transglutaminase + Ca: Time-resolved 63 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 18.0 nm
VolumePorod 155 nm3

SASDRH3 – Tissue Transglutaminase + Ca: Time-resolved 100 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 20.0 nm
VolumePorod 190 nm3

SASDRJ3 – Tissue Transglutaminase + Ca: Time-resolved 316 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 200 nm3

SASDRK3 – Tissue Transglutaminase + Ca: Time-resolved 631 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.6 nm
Dmax 26.0 nm
VolumePorod 215 nm3

SASDRL3 – Tissue Transglutaminase + Ca: Time-resolved 1500 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.6 nm
Dmax 28.0 nm
VolumePorod 230 nm3

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.0 nm
Dmax 27.0 nm
VolumePorod 241 nm3

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 300 nm3

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 6.9 nm
Dmax 31.5 nm
VolumePorod 490 nm3

SASDRQ3 – Trypsin + Aprotinin: Time-Resolved 10 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 23 nm3

SASDRR3 – Trypsin + Aprotinin: Time-Resolved 30 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDRS3 – Trypsin + Aprotinin: Time-Resolved 100 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 30 nm3

SASDRT3 – Trypsin + Aprotinin: Time-Resolved 400 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 27 nm3

SASDRU3 – Trypsin + Aprotinin: Time-Resolved 630 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 25 nm3

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.0 nm
Dmax 5.3 nm
VolumePorod 29 nm3

SASDRX3 – Trypsin + Aprotinin: Complex equilibrium

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 31 nm3

SASDRY3 – Kenics GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 28 nm3

SASDRZ3 – Kenics GAC rRNA + Mg: Time-Resolved 32 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 27 nm3

SASDR24 – Kenics GAC rRNA + Mg: Time-Resolved 63 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 9.5 nm
VolumePorod 27 nm3

SASDR34 – Kenics GAC rRNA + Mg: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 28 nm3

SASDR44 – Kenics GAC rRNA + Mg: Time-Resolved 316 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 27 nm3

SASDR54 – Kenics GAC rRNA + Mg: Time-Resolved 631 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.3 nm
Dmax 9.8 nm
VolumePorod 26 nm3

SASDR64 – Kenics GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.3 nm
Dmax 11.0 nm
VolumePorod 26 nm3

SASDR74 – Kenics GAC rRNA + Mg: Equilibrium initial

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 32 nm3

SASDR84 – Kenics GAC rRNA + Mg: Equilibrium final

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 26 nm3

SASDR94 – GAC rRNA + L11 Protein: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 12.0 nm
VolumePorod 34 nm3

SASDRA4 – GAC rRNA + L11 Protein: Time-Resolved 50 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 13.5 nm
VolumePorod 36 nm3

SASDRB4 – GAC rRNA + L11 Protein: Time-Resolved 63 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 33 nm3

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 35 nm3

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 37 nm3

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 13.0 nm
VolumePorod 38 nm3

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 40 nm3

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 12.8 nm
VolumePorod 39 nm3

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 13.0 nm
VolumePorod 48 nm3

SASDRK4 – Diffusive GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDRL4 – Diffusive GAC rRNA + Mg: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDRM4 – Diffusive GAC rRNA + Mg: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 9.0 nm
VolumePorod 29 nm3

SASDRN4 – Diffusive GAC rRNA + Mg: Time-Resolved 300 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 13.0 nm
VolumePorod 30 nm3

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 34 nm3

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDBS7 – Nucleosome Core Particle: 1.2 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.2 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pabit SA, Gloss LM, Pollack L
RgGuinier 5.8 nm

SASDBT7 – Nucleosome Core Particle: 1.9 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.9 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pabit SA, Gloss LM, Pollack L
RgGuinier 6.8 nm

SASDJV7 – pri-miR16-1 primary microRNA

primary microRNA pri-miR16-1 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 74 nm3

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3