Molecular model of a sensor of two-component signaling system

Ryzhykau Y, Orekhov P, Rulev M, Vlasov A, Melnikov I, Volkov D, Nikolaev M, Zabelskii D, Murugova T, Chupin V, Rogachev A, Gruzinov A, Svergun D, Brennich M, Gushchin I, Soler-Lopez M, Bothe A, Büldt G, Leonard G, Engelhard M, Kuklin A, Gordeliy V
Scientific Reports 11(1) (2021 May 24)

doi: 10.1038/s41598-021-89613-6

Submitted to SASBDB: 2021 Jan 4 Published in SASBDB: 2021 May 4

SASDKZ6 – SAXS data for the complex of the sensory rhodopsin II with its cognate truncated transducer in detergent at 0.15 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Truncated sensory rhodopsin II transducer containing transmembrane and HAMP1 domains dimer, 30 kDa Natronomonas pharaonis protein Buffer: 150 mM NaCl, 25 Na/K-Pi, 1 mM EDTA, 0.05 % DDM, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 13 RgGuinier 4.8 nm Dmax 14.0 nm

SASDK27 – SAXS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 9 RgGuinier 9.0 nm Dmax 38.9 nm

SASDK37 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25 RgGuinier 7.1 nm Dmax 35.0 nm

SASDK47 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 1.4 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein Buffer: 1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10 RgGuinier 9.9 nm Dmax 36.5 nm

SASDK57 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 2.8 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein Buffer: 2800 mM NaCl, 76.6 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10 RgGuinier 9.3 nm Dmax 37.5 nm

SASDK67 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 4.0 M NaCl Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein Buffer: 4000 mM NaCl, 100 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25 RgGuinier 8.9 nm Dmax 39.0 nm