Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.

Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y
iScience 24(5):102476 (2021 May 21)
PMID: 34113820
doi: 10.1016/j.isci.2021.102476 		Submitted to SASBDB: 2021 Jan 25
Published in SASBDB:

SASDK48 – Histidine kinase AdeS - DHp-CA Domain

Histidine kinase experimental SAS data
MULTIFOXS model
Sample: Histidine kinase dimer, 51 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Apr 21
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDK58 – Histidine kinase AdeS - cytoplasmic domain

Histidine kinase experimental SAS data
OTHER model
Sample: Histidine kinase hexamer, 186 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 19
RgGuinier 4.6 nm
Dmax 16.3 nm
VolumePorod 378 nm3