Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.

Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y, iScience 24(5):102476 (2021) Europe PMC

SASDK58 – Histidine kinase AdeS - cytoplasmic domain

Histidine kinase
MWexperimental 209 kDa
MWexpected 186 kDa
VPorod 378 nm3
log I(s) 1.97×102 1.97×101 1.97×100 1.97×10-1
Histidine kinase small angle scattering data  s, nm-1
ln I(s)
Histidine kinase Guinier plot ln 1.98×102 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Histidine kinase Kratky plot 1.104 0 √3 sRg
p(r)
Histidine kinase pair distance distribution function Rg: 4.7 nm 0 Dmax: 16.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Histidine kinase OTHER model
Synchrotron SAXS data from solutions of the cytoplasmic domain of histidine kinase AdeS in 20 mM Tris, 150 mM NaCl, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histidine kinase (AdeS)
Mol. type   Protein
Organism   Acinetobacter baumannii
Olig. state   Hexamer
Mon. MW   31.0 kDa
 
UniProt   Q93E22 (84-357)
Sequence   FASTA