Conformational landscape of multidomain SMAD proteins

Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
Computational and Structural Biotechnology Journal (2021 Sep)

doi: 10.1016/j.csbj.2021.09.009

Submitted to SASBDB: 2021 Feb 23 Published in SASBDB: 2021 Sep 14

SASDLA2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 0.5 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant), 53 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4 RgGuinier 5.1 nm Dmax 20.0 nm

SASDLB2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 1 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant), 53 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4 RgGuinier 5.2 nm Dmax 20.0 nm

SASDLC2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 1.5 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant), 53 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4 RgGuinier 5.3 nm Dmax 20.0 nm

SASDKZ8 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 0.74 mg/ml Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 3.4 nm Dmax 15.0 nm

SASDK29 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 1.3 mg/ml Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 3.8 nm Dmax 21.0 nm

SASDK39 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 2.5 mg/ml Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 3.8 nm Dmax 20.0 nm

SASDK49 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 3mg/ml Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 4.3 nm Dmax 23.0 nm

SASDK59 – Mothers against decapentaplegic homolog 2, SMAD2, linker fragment (amino acids 175-262) Sample: Mothers against decapentaplegic homolog 2 (linker) monomer, 10 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 2.9 nm Dmax 14.0 nm VolumePorod 20 nm3

SASDK69 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 0.5 mg/ml Sample: Mothers against decapentaplegic homolog 2 (linker-MH2), 99 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 3.6 nm Dmax 15.0 nm

SASDK79 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 1.1 mg/ml Sample: Mothers against decapentaplegic homolog 2 (linker-MH2), 99 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 3.7 nm Dmax 17.0 nm

SASDK89 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 2.2 mg/ml Sample: Mothers against decapentaplegic homolog 2 (linker-MH2), 99 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 4.2 nm Dmax 19.0 nm

SASDK99 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 1 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion), 158 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 4.1 nm Dmax 17.0 nm

SASDKA9 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 2 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion), 158 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 4.3 nm Dmax 20.0 nm

SASDKB9 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 3.8 mg/ml Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion), 158 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11 RgGuinier 4.6 nm Dmax 22.0 nm

SASDKC9 – Mothers against decapentaplegic homolog 4, SMAD4, wild-type Sample: Mothers against decapentaplegic homolog 4 monomer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 4 RgGuinier 4.4 nm Dmax 22.0 nm

SASDKD9 – Mothers against decapentaplegic homolog 4, SMAD4, linker fragment (amino acids 141-271) Sample: Mothers against decapentaplegic homolog 4 (linker) monomer, 14 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20 RgGuinier 3.7 nm Dmax 17.5 nm VolumePorod 45 nm3

SASDKE9 – Mothers against decapentaplegic homolog 4, SMAD4, linker-MH2 fragment (amino acids 150-552) Sample: Mothers against decapentaplegic homolog 4 (linker-MH2) monomer, 44 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20 RgGuinier 3.3 nm Dmax 17.0 nm VolumePorod 80 nm3

SASDKF9 – Mothers against decapentaplegic homolog 4, SMAD4, SADMH2 fragment (amino acids 272-552) Sample: Mothers against decapentaplegic homolog 4 (SADMH2) monomer, 31 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 54 nm3

SASDKG9 – Mothers against decapentaplegic homolog 4, SMAD4, MH2 fragment (amino acids 314-552) Sample: Mothers against decapentaplegic homolog 4 (MH2 Fragment) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20 RgGuinier 2.1 nm Dmax 8.0 nm

SASDKH9 – Mothers against decapentaplegic homolog 4, SMAD4, MH1 fragment (amino acids 10-140) Sample: Mothers against decapentaplegic homolog 4 (MH1 fragment) monomer, 15 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20 RgGuinier 1.6 nm Dmax 6.0 nm VolumePorod 24 nm3