Structural basis for the inhibition of the Bacillus subtilis c-di-AMP cyclase CdaA by the phosphoglucomutase GlmM

Pathania M, Tosi T, Millership C, Hoshiga F, Morgan R, Freemont P, Gründling A
Journal of Biological Chemistry :101317 (2021 Oct)

doi: 10.1016/j.jbc.2021.101317

Submitted to SASBDB: 2021 May 13 Published in SASBDB: 2021 Oct 18

SASDL25 – Bacillus subtilis complex of diadenylate cyclase (CdaA:cytoplasmic domain) and phosphoglucosamine mutase (GlmM) Sample: Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 21 RgGuinier 4.5 nm Dmax 16.2 nm VolumePorod 250 nm3

SASDMQ5 – Bacillus subtilis complex of diadenylate cyclase (CdaA:cytoplasmic domain) in complex with a truncated version of phosphoglucosamine mutase (GlmMF369) Sample: Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein Phosphoglucosamine mutase dimer, 83 kDa Bacillus subtilis protein Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Aug 2 RgGuinier 3.7 nm Dmax 15.1 nm VolumePorod 156 nm3

SASDLZ4 – Bacillus subtilis phosphoglucosmine mutase GlmM Sample: Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 21 RgGuinier 3.7 nm Dmax 12.2 nm VolumePorod 140 nm3

SASDLY4 – Bacillus subtilis diadenylate cyclase CdaA: cytoplasmic domain Sample: Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 21 RgGuinier 2.6 nm Dmax 8.9 nm VolumePorod 60 nm3