Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit

Piper S, Edwards M, van Wonderen J, Casadevall C, Martel A, Jeuken L, Reisner E, Clarke T, Butt J
Frontiers in Microbiology 12 (2021 Aug 16)

doi: 10.3389/fmicb.2021.714508
Submitted to SASBDB: 2021 Jun 14
Published in SASBDB:

SASDL97 – Ru-MtrCAB complex formed by reconstitution of Ru-MtrC and MtrAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrAExtracellular iron oxide respiratory system surface decaheme cytochrome c component MtrCExtracellular iron oxide respiratory system outer membrane component MtrB experimental SAS data
DAMMIN model
Sample: Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 77 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment: SANS data collected at D22, ILL on 2020 Jan 13
RgGuinier 4.7 nm
Dmax 16.6 nm
VolumePorod 154 nm3

SASDLA7 – Outer membrane protein complex MtrCAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrAExtracellular iron oxide respiratory system outer membrane component MtrBExtracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC experimental SAS data
DAMMIN model
Sample: Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment: SANS data collected at D22, ILL on 2016 Sep 12
RgGuinier 5.2 nm
Dmax 17.1 nm
VolumePorod 234 nm3