A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.

McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
Nat Commun 13(1):4105 (2022 Jul 14)

PMID: 35835745 doi: 10.1038/s41467-022-31678-6

Submitted to SASBDB: 2021 Jul 9 Published in SASBDB: 2022 Jun 2

SASDMK3 – Mycobacterium phage TipsytheTRex apo protein Sample: Immunity repressor monomer, 24 kDa Mycobacterium phage TipsytheTRex protein Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10 RgGuinier 2.3 nm Dmax 9.0 nm VolumePorod 43 nm3

SASDML3 – Mycobacterium phage TipsytheTRex immunity repressor bound to 24mer DNA Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 24mer dsDNA monomer, 15 kDa DNA Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10 RgGuinier 2.4 nm Dmax 9.0 nm VolumePorod 43 nm3

SASDMM3 – Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 13mer dsDNA monomer, 8 kDa DNA Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 10 RgGuinier 2.0 nm Dmax 8.5 nm VolumePorod 18 nm3

SASDLS7 – Mycobacterium phage TipsytheTRex immunity repressor bound to DNA Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 21mer dsDNA monomer, 13 kDa DNA Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10 RgGuinier 2.3 nm Dmax 7.4 nm VolumePorod 41 nm3