A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.

McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR, Nat Commun 13(1):4105 (2022) Europe PMC

SASDMK3 – Mycobacterium phage TipsytheTRex apo protein

Immunity repressor
MWexperimental 17 kDa
MWexpected 24 kDa
VPorod 43 nm3
log I(s) 8.67×100 8.67×10-1 8.67×10-2 8.67×10-3
Immunity repressor small angle scattering data  s, nm-1
ln I(s)
Immunity repressor Guinier plot ln 8.67×100 Rg: 2.3 nm 0 (2.3 nm)-2 s2
Immunity repressor Kratky plot 1.104 0 3 sRg
Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Immunity repressor BILBOMD model

Synchrotron SAXS data from solutions of Mycobacterium phage TipsytheTRex apo protein in 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Immunity repressor
Mol. type   Protein
Organism   Mycobacterium phage TipsytheTRex
Olig. state   Monomer
Mon. MW   23.6 kDa
UniProt   A0A2D1GKF7 (1-183)
Sequence   FASTA