K -edge anomalous SAXS for protein solution structure modeling

Virk K, Yonezawa K, Choukate K, Singh L, Shimizu N, Chaudhuri B
Acta Crystallographica Section D Structural Biology 78(2) (2022 Jan 21)

doi: 10.1107/S205979832101247X

Submitted to SASBDB: 2021 Nov 9 Published in SASBDB: 2022 Jan 25

SASDMY7 – Poly-histidine tagged Myosin X component at 8979 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.5 nm VolumePorod 24 nm3

SASDMZ7 – Poly-histidine tagged Myosin X component at 8981 eV Sample: Unconventional myosin-X dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM28 – Poly-histidine tagged Myosin X component at 8983 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM38 – Poly-histidine tagged Myosin X component at 8985 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM48 – Poly-histidine tagged Myosin X component at 8987 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM58 – Poly-histidine tagged Myosin X component at 8989 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM68 – Poly-histidine tagged Myosin X component at 8991 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 24 nm3

SASDM78 – Poly-histidine tagged Myosin X component at 8993 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.2 nm Dmax 8.0 nm

SASDM88 – Poly-histidine tagged Myosin X component with CuSO4 at 8979 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 10.5 nm VolumePorod 27 nm3

SASDM98 – Poly-histidine tagged Myosin X component with CuSO4 at 8981 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.5 nm Dmax 11.5 nm VolumePorod 27 nm3

SASDMA8 – Poly-histidine tagged Myosin X component with CuSO4 at 8983 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 10.5 nm VolumePorod 27 nm3

SASDMB8 – Poly-histidine tagged Myosin X component with CuSO4 at 8985 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.5 nm Dmax 11.5 nm VolumePorod 27 nm3

SASDMC8 – Poly-histidine tagged Myosin X component with CuSO4 at 8987 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 11.0 nm VolumePorod 28 nm3

SASDMD8 – Poly-histidine tagged Myosin X component with CuSO4 at 8989 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 11.5 nm VolumePorod 27 nm3

SASDME8 – Poly-histidine tagged Myosin X component with CuSO4 at 8991 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 11.5 nm VolumePorod 28 nm3

SASDMF8 – Poly-histidine tagged Myosin X component with CuSO4 at 8993 eV Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16 RgGuinier 2.4 nm Dmax 11.5 nm VolumePorod 28 nm3