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75 hits found for Chaudhuri

SASDEB2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73E

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 19.8 nm
Dmax 23.4 nm

SASDTB2 – WAXS data of poly-histidine tagged Ribose binding protein (16 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDEC2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73A

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 21.8 nm
Dmax 13.6 nm

SASDTC2 – WAXS data of poly-histidine tagged Ribose binding protein (20 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDTD2 – WAXS data of poly-histidine tagged Ribose binding protein (17 mg/mL) from Escherichia coli in the presence of 5 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: Tris 50 mM, 50 mM NaCl, 10% glycerol, 5 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 1.9 nm
Dmax 10.7 nm
VolumePorod 25 nm3

SASDFF2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.5 nm

SASDFG2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 11.4 nm

SASDFH2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.5 nm

SASDFJ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.1 nm

SASDFK2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.9 nm

SASDFL2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.0 nm

SASDFM2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.8 nm

SASDFN2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.2 nm

SASDFP2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 8.1 nm

SASDFQ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 8.1 nm

SASDFR2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 15.2 nm

SASDFS2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 13.2 nm

SASDFT2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 15.3 nm

SASDFU2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 14.5 nm

SASDFV2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 12.1 nm

SASDFW2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 13.1 nm

SASDFX2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 11.1 nm

SASDFY2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 310-314)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.6 nm

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.2 nm

SASDHH4 – N-terminal domain of Wag31 from M. tuberculosis

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 tetramer, 34 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 3
Structural basis of self-assembly in the lipid-binding domain of mycobacterial polar growth factor Wag31 IUCrJ 7(4):767-776 (2020)
...Chaudhuri B
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 52 nm3

SASDR37 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin

HOTag-GS-Ubiquitin experimental SAS data
HOTag-GS-Ubiquitin Kratky plot
Sample: HOTag-GS-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 74 nm3

SASDR47 – HOTag6 tetramerization domain followed by a (Gly-Ser)2 linker fused to human monoubiquitin

HOTag6-(GS)2-Ubiquitin experimental SAS data
HOTag6-(GS)2-Ubiquitin Kratky plot
Sample: HOTag6-(GS)2-Ubiquitin tetramer, 52 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 13.7 nm
VolumePorod 69 nm3

SASDR57 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin

HOTag-(GS)4-Ubiquitin experimental SAS data
HOTag-(GS)4-Ubiquitin Kratky plot
Sample: HOTag-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 14.3 nm
VolumePorod 76 nm3

SASDR67 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin

HOTag-(GS)10-Ubiquitin experimental SAS data
HOTag-(GS)10-Ubiquitin Kratky plot
Sample: HOTag-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.0 nm
Dmax 14.8 nm
VolumePorod 84 nm3

SASDR77 – HOTag6 tetramerization domain followed by a (Gly-Ser)25 linker fused to human monoubiquitin

HOTag6-(GS)25-Ubiquitin experimental SAS data
HOTag6-(GS)25-Ubiquitin Kratky plot
Sample: HOTag6-(GS)25-Ubiquitin tetramer, 65 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.7 nm
Dmax 19.3 nm
VolumePorod 117 nm3

SASDR87 – HOTag6 tetramerization domain followed by a (Gly-Ser)50 linker fused to human monoubiquitin

HOTag-(GS)50-Ubiquitin experimental SAS data
HOTag-(GS)50-Ubiquitin Kratky plot
Sample: HOTag-(GS)50-Ubiquitin tetramer, 80 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.6 nm
Dmax 22.7 nm
VolumePorod 193 nm3

SASDR97 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin

HOTag-PA-Ubiquitin experimental SAS data
HOTag-PA-Ubiquitin Kratky plot
Sample: HOTag-PA-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 73 nm3

SASDRA7 – HOTag6 tetramerization domain followed by a (Pro-Ala)2 linker fused to human monoubiquitin

HOTag6-(PA)2-Ubiquitin experimental SAS data
HOTag6-(PA)2-Ubiquitin Kratky plot
Sample: HOTag6-(PA)2-Ubiquitin tetramer, 52 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 74 nm3

SASDRB7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin

HOTag-(PA)4-Ubiquitin experimental SAS data
HOTag-(PA)4-Ubiquitin Kratky plot
Sample: HOTag-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 78 nm3

SASDRC7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 18.4 nm
VolumePorod 106 nm3

SASDRD7 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)-Ubiquitin experimental SAS data
HOTag6-(GS)-Ubiquitin Kratky plot
Sample: HOTag6-(GS)-Ubiquitin tetramer, 51 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 70 nm3

SASDRE7 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)4-Ubiquitin experimental SAS data
HOTag6-(GS)4-Ubiquitin Kratky plot
Sample: HOTag6-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 12.8 nm
VolumePorod 114 nm3

SASDRF7 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)10-Ubiquitin experimental SAS data
HOTag6-(GS)10-Ubiquitin Kratky plot
Sample: HOTag6-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.2 nm
VolumePorod 86 nm3

SASDRG7 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)-Ubiquitin experimental SAS data
HOTag6-(PA)-Ubiquitin Kratky plot
Sample: HOTag6-(PA)-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.4 nm
VolumePorod 71 nm3

SASDRH7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)4-Ubiquitin experimental SAS data
HOTag6-(PA)4-Ubiquitin Kratky plot
Sample: HOTag6-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.7 nm
VolumePorod 80 nm3

SASDRJ7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)10-Ubiquitin experimental SAS data
HOTag6-(PA)10-Ubiquitin Kratky plot
Sample: HOTag6-(PA)10-Ubiquitin tetramer, 57 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 105 nm3

SASDRK7 – M1-linked tetraubiquitin, M1(1-72)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 41 nm3

SASDRL7 – M1-linked tetraubiquitin, M1(1-73)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.3 nm
Dmax 12.8 nm
VolumePorod 41 nm3

SASDRM7 – M1-linked tetraubiquitin, M1(1-74)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.2 nm
VolumePorod 43 nm3

SASDRN7 – M1-linked tetraubiquitin Pro-Ser/Gly-Ser fusion, M1(1-76)-PS(GS)4-Ub4

Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 experimental SAS data
Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 Kratky plot
Sample: Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 monomer, 36 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.4 nm
Dmax 13.9 nm
VolumePorod 50 nm3

SASDRP7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)3A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub monomer, 39 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.2 nm
Dmax 18.2 nm
VolumePorod 62 nm3

SASDRQ7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)6A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub monomer, 43 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.2 nm
Dmax 21.2 nm
VolumePorod 108 nm3

SASDMY7 – Poly-histidine tagged Myosin X component at 8979 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 24 nm3

SASDMZ7 – Poly-histidine tagged Myosin X component at 8981 eV

Unconventional myosin-X experimental SAS data
Unconventional myosin-X Kratky plot
Sample: Unconventional myosin-X dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM28 – Poly-histidine tagged Myosin X component at 8983 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM38 – Poly-histidine tagged Myosin X component at 8985 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM48 – Poly-histidine tagged Myosin X component at 8987 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM58 – Poly-histidine tagged Myosin X component at 8989 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM68 – Poly-histidine tagged Myosin X component at 8991 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM78 – Poly-histidine tagged Myosin X component at 8993 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm

SASDM88 – Poly-histidine tagged Myosin X component with CuSO4 at 8979 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 10.5 nm
VolumePorod 27 nm3

SASDM98 – Poly-histidine tagged Myosin X component with CuSO4 at 8981 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDMA8 – Poly-histidine tagged Myosin X component with CuSO4 at 8983 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 10.5 nm
VolumePorod 27 nm3

SASDMB8 – Poly-histidine tagged Myosin X component with CuSO4 at 8985 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDMC8 – Poly-histidine tagged Myosin X component with CuSO4 at 8987 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 28 nm3

SASDMD8 – Poly-histidine tagged Myosin X component with CuSO4 at 8989 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDME8 – Poly-histidine tagged Myosin X component with CuSO4 at 8991 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 28 nm3

SASDMF8 – Poly-histidine tagged Myosin X component with CuSO4 at 8993 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 28 nm3

SASDRK8 – HOTag6 tetramerization domain followed by a (Pro-Ala)25 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 6.3 nm
Dmax 24.9 nm
VolumePorod 180 nm3

SASDSB9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.6 nm
VolumePorod 35 nm3

SASDSC9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.4 nm
VolumePorod 37 nm3

SASDSD9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 39 nm3

SASDSE9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.0 nm
Dmax 8.3 nm
VolumePorod 35 nm3

SASDSF9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 36 nm3

SASDSG9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 39 nm3

SASDEU7 – Stator protein FlaG soluble domain

Conserved flagellar protein FlaG soluble domain experimental SAS data
Conserved flagellar protein FlaG soluble domain Kratky plot
Sample: Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 133 nm3

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 109 nm3

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y

Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain experimental SAS data
BILBOMD model
Sample: Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 90 nm3

SASDET7 – Stator protein complex FlaG-V118K/FlaF

Conserved flagellar protein FStator protein FlaG-V118K soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 108 nm3