Mg²⁺-dependent conformational equilibria in CorA and an integrated view on transport regulation.

Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
Elife 11 (2022 Feb 7)

PMID: 35129435 doi: 10.7554/eLife.71887

Submitted to SASBDB: 2021 Aug 4 Published in SASBDB: 2021 Nov 29

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+ Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23 RgGuinier 4.2 nm Dmax 13.9 nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+ Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23 RgGuinier 4.3 nm Dmax 13.9 nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+ Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23 RgGuinier 4.8 nm Dmax 14.2 nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+ Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23 RgGuinier 4.9 nm Dmax 14.3 nm