The low-resolution structural models of hepatitis C virus RNA subdomain 5BSL3.2 and its distal complex with domain 3'X point to conserved regulatory mechanisms within the Flaviviridae family.

Castillo-Martínez J, Fan L, Szewczyk MP, Wang YX, Gallego J
Nucleic Acids Res (2022 Feb 7)
PMID: 35137150
doi: 10.1093/nar/gkac061
Submitted to SASBDB: 2021 Sep 6
Published in SASBDB:

SASDMM4 – Hepatitis C virus 5BSL3.2 RNA subdomain

5BSL3.2 experimental SAS data
DAMFILT model
Sample: 5BSL3.2 monomer, 15 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 0.1 mM EDTA, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2019 Jun 2
RgGuinier 2.5 nm
Dmax 7.6 nm
VolumePorod 29 nm3

SASDMN4 – Hepatitis C virus 5BSL3.2 RNA subdomain

5BSL3.2 experimental SAS data
DAMFILT model
Sample: 5BSL3.2 monomer, 15 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 6 mM MgCl2, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2019 Jun 2
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDMP4 – Hepatitis C virus RNA Domain 3'X mutant

Domain 3'X mutant experimental SAS data
DAMFILT model
Sample: Domain 3'X mutant monomer, 32 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 0.1 mM EDTA, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2019 Jun 2
RgGuinier 4.1 nm
Dmax 14.7 nm
VolumePorod 70 nm3

SASDMQ4 – Hepatitis C virus RNA Domain 3'X mutant

Domain 3'X mutant experimental SAS data
DAMFILT model
Sample: Domain 3'X mutant monomer, 32 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 6 mM MgCl2, pH: 7
Experiment: SAXS data collected at 12ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 2
RgGuinier 3.3 nm
Dmax 14.2 nm
VolumePorod 50 nm3

SASDMR4 – Hepatitis C virus RNA Domain 3'X mutant - Subdomain 5BSL3.2 complex

Domain 3'X mutant - 5BSL3.2 complex experimental SAS data
DAMFILT model
Sample: Domain 3'X mutant - 5BSL3.2 complex monomer, 42 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 6 mM MgCl2, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2019 Jun 2
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 88 nm3